xCELLigence System (Roche Applied / ACEA Biosciences Inc.) is a routinely used platform in in vitro services for monitoring cellular events real time, without the use of labels (label-free). The System measures electrical impedance through interdigitated micro-electrodes integrated in the bottom of tissue culture e-Plates. Impedance measurement provides quantitative information about the biological status of the cells, including cell number, viability, and morphology. A wide range of cell-based assays for both high throughput screening and research laboratory environments can be performed on xCELLigence System.
We optimized the screening strategy based on xCELLigence system to screen cytoprotective compounds. This method has been published and validated.